rabbit polyclonal antibody against cd163  (Biospes Inc)

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: CD163 immunostaining showed that ( A ) PDAC Tumor cells were negative (IHCx40) ( B ) High expressions of CD163 in TAM in PDAC (IHCx200) ( C ) High expressions of CD163 in TAM in PDAC (IHCx400) ( D ) Negative expressions in control pancreatic tissue (IHCx200)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Immunostaining, Control

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Comparison between PDAC and control groups regarding CD163 staining

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Comparison, Control

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: High H score values of CD163 staining showed significant associations with ( A ) higher histological grade ( p < 0.026) ( B ) T3 tumor stage ( p < 0.002) ( C ) advanced stage group ( p < 0.001) ( D ) larger tumor size ( p < 0.002) ( E ) Significant direct correlation between high mean H score value of CD163 staining with larger tumor size ( p < 0.031)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Staining

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Relationships between HOXA9 and CD163 in PDAC ( n = 98)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques:

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Shorter overall survival by univariate survival analysis of PDAC cases showed significant associations with ( A ) Positive virology (HCV infection) ( P < 0.040) ( B ) high direct bilirubin ( P < 0.046) ( C ) larger tumor size ( P < 0.046) ( D ) advanced stage ( P < 0.034) (( E ) high median H score of HOXA9 ( P < 0.015) and CD163 ( P < 0.042)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Infection

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Multivariate COX regression analysis for the parameters affecting overall survival

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Infection

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Multivariate COX regression analysis for the parameters affecting overall survival (continuous variables)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques:

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Univariate and multivariate COX regression analysis for the parameters affecting overall survival in negative Virology (HCV) cases

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques:

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Univariate overall survival of PDAC cases (53 cases)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Infection

Journal: Frontiers in Immunology

Article Title: The Antimicrobial Peptide Human β-Defensin-3 Accelerates Wound Healing by Promoting Angiogenesis, Cell Migration, and Proliferation Through the FGFR/JAK2/STAT3 Signaling Pathway

doi: 10.3389/fimmu.2021.712781

Figure Lengend Snippet: hBD-3 accelerates the inflammatory phase of wound healing and decreases inflammatory responses. Representative images of skin wound biopsies from mice treated with 0.01% acetic acid (vehicle) and 200 µg/ml hBD-3. On day 2 and day 4 post-injury, sections were immunohistochemically stained with (A) anti-myeloperoxidase (MPO) antibody for neutrophil detection, (B) with anti-Iba-1 antibody for macrophage detection, and (C) with anti-CD80 (upper panels) and anti-CD163 (lower panels) antibodies for M1 and M2 macrophage detection followed by counterstaining with hematoxylin. MPO-, Iba-1- and CD163-positive cells were detected in both the epidermis and dermis of the wounds. Original magnification: 20×. Number of MPO-, Iba-1- and CD163-positive cells were counted and shown in right panels. The P value was calculated using Student’s t test. * P < 0.05 for comparisons between the vehicle-treated and hBD-3-treated wounds. n = 3.

Article Snippet: Rabbit polyclonal antibody against CD163 was obtained from Proteintech (Rosemont, IL).

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: Beneficial Effects of Melatonin on Apolipoprotein-E Knockout Mice by Morphological and 18 F-FDG PET/CT Assessments

doi: 10.3390/ijms21082920

Figure Lengend Snippet: Immunohistochemical characterization of macrophage population. Labelling of the aorta’s wall with CD68 ( a , b ) and CD163 ( d , e ) antibodies. Each panel shows a full-field image at 1000× (scale bars: 20 µm). Immunohistochemical photomicrograph shows the positivity for CD68, mainly localized in the subendothelial space and in the tunica adventitia of control mice ( a ). In contrast, the immunohistochemical photomicrograph of ApoE -/- mice treated with melatonin highlights that this immunopositivity slightly decreased in the subendothelial space, even if not significantly, after melatonin supplementation ( b ). CD163 expression was negative or really weak in control mice ( d ). In contrast, the photomicrograph of MLT-treated mice showed that CD163 was moderately/strongly expressed both in the subendothelial space and tunica adventitia, increasing its positivity after melatonin administration ( e ). Graphs summarize the number of CD68 ( c ) and CD163 ( f ) positive cells, obtained for both macrophage markers, evaluating five non-overlapping fields with the same area from three non-consecutive aorta sections. For both CD68 ( g ) and CD163 ( h ), sections without primary antibody and in the presence of isotype matched immunoglobulins G served as negative immunohistochemical controls. Statistical analyses comparing multiple continuous outcomes were performed using one-way analyses of variance test corrected by Bonferroni for immunomorphometrical evaluations. Continuous variables are summarized as means ± standard deviation. Error bars represent the 95% confidence interval around the mean; * indicates the level of significance, p ≤ 0.05; black arrow indicates CD68 positive macrophage; black arrowhead indicates CD163 positive M2 macrophage; I, tunica intima; M, tunica media; A, tunica adventitia; AP, atherosclerotic plaque; CTR, control group; MLT, mice treated with melatonin.

Article Snippet: Alternate paraffin sections were dewaxed, rehydrated, and incubated in 3% hydrogen peroxide for 30 min. Then, after the blocking step in 3% bovine serum albumin solution for 1 h, the sections were incubated 1 h at 37 °C and 30 min at room temperature with the following primary antibodies: rat monoclonal antibody against CD68 (diluted 1:100; Abcam, Cambridge, UK); rabbit polyclonal antibody against CD163 (diluted 1:50; Abcam, Cambridge, UK); goat polyclonal antibody against TNF-α (diluted 1:200 Santa Cruz Biotechnology Inc., Dallas, TX, USA) [ ]; mouse monoclonal antibody against TGF-β (diluted 1:150; Santa Cruz Biotechnology Inc., Dallas, TX, USA) [ ] and simultaneously with mouse monoclonal antibody against ICAM-1 (diluted 1:200; Santa Cruz Biotechnology Inc., Dallas, TX, USA) and rabbit polyclonal antibody against VCAM-1 (diluted 1:200; Santa Cruz Biotechnology Inc., Dallas, TX, USA).

Techniques: Immunohistochemical staining, Control, Expressing, Standard Deviation